Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell. Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence. Epigenomic maintenance is a continuous process and plays an important role in stability of eukaryotic genomes by taking part in crucial biological mechanisms like DNA repair. Plant flavones are said to be inhibiting epigenomic marks that cause cancers. Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis. The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays.
For didactic purposes, epigenetic modifications can be grouped into three main categories: DNA methylation, histone modifications and nucleosome positioning (Portela and Esteller, 2010). An altered pattern of epigenetic modifications is central to many common human diseases, including cancer (Esteller, 2007; Esteller, 2008).
Including:
- DNA modification analysis
- DNA methylation array analysis
- Enrichment Analysis,BS-seq analysis
Including:
- Nucleosome positioning
- MNase-seq analysis,
- ATAC-seq analysis
Including:
- HT-SELEX Analysis
- DNase-seq Analysis
- ChIO-seq Analysis